嫦娥医学肽度酒研究报告人陈希博士
陈希，男，博士，高级工程师，毕业于武汉大学生命科学学院，在校期间搭建了武汉大学第一个生物质谱平台，从事蛋白质组学研究工作。毕业后前往香港大学任职高级研究助理，继续从事生物质谱方向研究。2013年，前往武汉生物技术研
究院，创建了生物质谱平台，负责平台的技术及管理运营，同时作为武汉生物技术研究院建院以来的第一个博士后，进行深造。2015年底，陈希博士荣获由湖北省人力资源和社会保障厅颁发的“湖北省企业研发人才”称号。2017年底，陈希博士依托武汉生物技术研究院创办了谱度众合（武汉）生命科技有限公司，担任公司总经理，从事：（1）蛋白质组学科技服务、（2）医学检测试剂盒研发。陈希博士在生物质谱及蛋白质组学领域拥有14年的科研和市场经验，发表SCI论文30余篇，申请国家发明专利9项，实用新型专利2项，软件著作权10项，并主持：（1）国家自然科学基金青年基金、（2）湖北省博士后创新岗位基金、（3）武汉市博士后科研启动基金。
教育背景
2004---2010 博士学位 武汉大学生命科学学院 生物化学与分子生物学系
2000---2004 学士学位 武汉大学生命科学学院 生物技术系
工作经历
2018---至今 总经理 谱度众合（武汉）生命科技有限公司
2013---2017 生物质谱平台主管 武汉生物技术研究院
2011---2013 高级研究助理 香港大学生物科学学院
主持项目
1. 国家自然科学基金青年科学基金；项目名称：错配修复介导的细胞凋亡相关蛋白的鉴定筛选及功；项目金额：24万元；主持人：陈希
2. 湖北省博士后创新岗位基金；项目名称：基于质谱技术的临床样本氨基酸快速检验；项目金额：10万元；主持人：陈希
3. 武汉市博士后科研启动基金；项目名称：临床样本氨基酸质谱检测试剂盒研发；项目金额：10万元；主持人：陈希
知识产权
1. 一种蛋白质凝胶样品的质谱检测前处理方法及处理试剂盒（发明专利；申请号：202010578728.8）
2. 一种磷酸化肽段富集装置及其富集方法（发明专利；申请号：201911265126.0）
3. 一种TNC蛋白检测试剂的应用及试剂盒（发明专利；申请号：202010749270.8）
4. 一种异构数据不依赖型蛋白质组学质谱分析系统及方法（发明专利；申请号：202011005330.1）
5. 一种改进的生物样本中磷酸化肽段的鉴定及定量方法（发明专利；申请号：201810287396.0）
6. 一种临床样本中游离氨基酸的定量检测方法（发明专利；申请号：201610786095.3）
7. 一种快速从微量血液中富集纯化维生素D的方法（发明专利；申请号：201911343590.7）
8. 一种血液或尿液中13种微量元素的质谱快速定量方法（发明专利；申请号：201911343932.5）
9. 一种磷酸化多肽富集的辅助装置（实用新型专利；授权：201821917095.3）
10. 一种用于肽段标记除盐的装置（实用新型专利；授权：201821684442.2）
11. 基于Uniprot和InterPro数据库的GO注释信息及映射关系整合软件V1.0（软件著作权；登记号：2018SR1005721）
12. GO层级数据库软件（软件著作权；登记号：2018SR1090409）
13. 基于ProteinPilot定量蛋白质组学数据处理软件V1.0（软件著作权；登记号：2018SR1090190）
14. 基于ProteinPilot定量磷酸化蛋白质组学数据处理软件V1.0（软件著作权；登记号：2018SR1090186）
15. 蛋白数据的GO和KEGG注释结果分析软件V1.0（软件著作权；登记号：2018SR979518）
16. 基于eggNOG5.0的COG注释结果富集软件V1.0（软件著作权；登记号：2020SR0257107）
17. 基于eggNOG4.5注释结果的COG注释结果整理软件V1.0（软件著作权；登记号：2020SR0256255）
18. 基于MaxQuant处理的LFQ差异蛋白多标准判定软件V1.0（软件著作权；登记号：2020SR0257103）
19. 基于ProteinPilot蛋白鉴定数据处理软件V1.0（软件著作权；登记号：2020SR0610586）
20. 基于MaxQuant蛋白鉴定数据处理软件V1.0（软件著作权；登记号：2020SR0713124）
发表论文
1. Chen X, Wu D, Zhao Y, Wong HB, Guo L: Increasing phosphoproteome coverage and identification of phosphorylation motifs through combination of different HPLC fractionation methods. J Chromatogr B 2011 Jan 1;879(1):25-34.
2. Chen X, Zhao Y, Li GM, Guo L: Proteomic analysis of mismatch repair-mediated alkylating agent-induced DNA damage response. Cell & Bioscience 2013 Sep 19;3(1):37.
3. Chen X, Chan WL, Zhu FY, Chu H, Chu IK, Lo C: Phosphoproteomic analysis of the non-seed vascular plant model Selaginella moellendorffii. Proteome Science 2014 Mar 17;12:16.
4. Zhu SL&, Chen X&, Wang LJ, Wan WW, Xin QL, Wang W, Xiao G, Zhang LK: Global quantitative proteomic analysis profiles host protein expression in response to Sendai virus infection. Proteomics 2017 Jan 9.
5. He XM, Chen X, Yuan BF, Feng YQ: Graft modification of cotton with phosphate group and its application to the enrichment of phosphopeptides. J Chromatogr A 2017 Feb 10;1484:49-57.
6. Hou D, Chen X, Zhang LK: Proteomic Analysis of Mamestra Brassicae Nucleopolyhedrovirus Progeny Virions from Two Different Hosts. PLoS One 2016 Apr 8; 11(4):e0153365.
7. Zhu GT, Chen X, He XM, Zhang Z, Li XS, Yuan BF, Feng YQ: Bioinspired preparation of monolithic ordered mesoporous silica for enrichment of endogenous peptides. RSC Advances 2015 Sep 1.
8. He XM, Chen X, Zhu GT, Wang Q, Yuan BF, Feng YQ: Hydrophilic Carboxyl Cotton Chelator for Titanium(IV) Immobilization and Its Application as Novel Fibrous Sorbent for Rapid Enrichment of Phosphopeptides. ACS Appl Mater Interfaces 2015 Aug 12;7(31):17356-62.
9. Zhu GT,
Chen X, He XM, Wang H, Zhang Z, Feng YQ: Electrospun Highly Ordered Mesoporous Silica–Carbon Composite Nanofibers for Rapid Extraction and Prefractionation of Endogenous Peptides. Chem Eur J 2015 Jan 30.10. Li XS, Chen X, Sun H, Yuan BF, Feng YQ: Perovskite for the highly selective enrichment of phosphopeptides. J Chromatogr A 2015 Jan 9.
11. Li XS, Chen X, Yuan BF, Feng YQ: Phosphonate-modified metal oxides for the highly selective enrichment of phosphopeptides. RSC Adv 2015, 5, 7832-7841.
12. Kang X, Chen X, He Y, Guo D, Guo L, Zhong J, Shu HB: DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus replication. Virology 2013 Jan 20;435(2):385-94.
13. Wang D, Gan G, Chen X, Zhong CQ: QuantPipe: A User-Friendly Pipeline Software Tool for DIA Data Analysis Based on the OpenSWATH-PyProphet-TRIC Workflow. J Proteome Res 2020 Oct 22.
14. Shu T, Ning W, Wu D, Xu J, Han Q, Huang M, Zou X, Yang Q, Yuan Y, Bie Y, Pan S, Mu J, Han Y, Yang X, Zhou H, Li R, Ren Y, Chen X, Yao S, Qiu Y, Zhang DY, Xue Y, Shang Y, Zhou X: Plasma Proteomics Identify Biomarkers and Pathogenesis of COVID-19. Immunity 2020 Oct 20;S1074-7613(20)30449-0.
15. Zhu FY, Song YC, Zhang KL, Chen X, Chen MX: Quantifying Plant Dynamic Proteomes by SWATH-based Mass Spectrometry. Trends in Plant Science 2020 September 2.
16. Chen MX, Lu CC, Sun PC, Nie YX, Tian Y, Hu QJ, Debatosh D, Hou XX, Gao B, Chen X, Liu SX, Zheng CC, Zhao XY, Dai L, Zhang J, Liu YG: Comprehensive transcriptome and proteome analyses reveal a novel sodium chloride responsive gene network in maize seed tissues during germination. Plant, Cell & Environment 2020 Jul 17.
17. Zhong CQ, Wu J, Qiu X, Chen X, Xie C, Han J: Generation of a murine SWATH-MS spectral library to quantify 11,000+ proteins. Sci Data 2020 Mar 26;7(1):104.
18. Chen MX, Zhu FY, Gao B, Ma KL, Zhang YJ, Fernie A, Chen X, Ye NH, Hu QJ, Tian Y, Zhang D, Liu TY, Zhang JH, Liu YG: Full-length transcript-based proteogenomics of rice improves its genome and proteome annotation. Plant Physiology 2020 March.
19. Zhong CQ, Wu R, Chen X, Wu S, Shuai J, Han J: Systematic assessment of the effect of internal library in targeted analysis of SWATH-MS. J Proteome Res 2020 Jan 3;19(1):477-492.
20. Wan W, Wang L, Chen X, Zhu S, Shang W, Xiao G, Zhang LK: A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells. Molecules 2019 Nov 20;24(23).
21. Chen MX, Sun C, Zhang KL, Song YC, Tian Y, Chen X, Liu YG, Ye NH, Zhang J, Qu S, Zhu FY: SWATH-MS-facilitated proteomic profiling of fruit skin between Fuji apple and a red skin bud sport mutant. BMC Plant Biol 2019 Oct 24;19(1):445.
22. Chen MX, Zhu FY, Wang FZ, Ye NH, Gao B, Chen X, Zhao SS, Fan T, Cao YY, Liu TY, Su ZZ, Xie LJ, Hu QJ, Wu HJ, Xiao S, Zhang J, Liu YG: Alternative splicing and translation play important roles in hypoxic germination in rice. J Exp Bot 2019 Feb 5;70(3):817-833.
23. Xiong T, Ye XD, Su YT, Chen X, Sun H, Li B, Chen YJ: Identification and Quantification of Proteins at Adsorption Layer of Emulsion Stabilized by Pea Protein Isolates. Colloids and Surfaces B: Biointerfaces 2018 May 30.
24. He XM, Liang XC, Chen X, Yuan BF, Zhou P, Zhang LN, Feng YQ: High Strength and Hydrophilic Chitosan Microspheres for the Selective Enrichment of N-Glycopeptides. Analytical Chemistry 2017 Sep 19;89(18):9712-9721.
25. Liu Y, Tan Z, Shu B, Zhang Y, Zheng C, Ke X, Chen X, Wang H, Zheng Z: An improved method for identifying SUMOylation sites of viral proteins. Virol Sin 2017 Dec;32(6):537-540.
26. Xin QL, Deng CL, Chen X, Wang J, Wang SB, Wang W, Deng F, Zhang B, Xiao G, Zhang LK: Quantitative Proteomic Analysis of Mosquito C6/36 Cells Reveals Host Proteins Involved in Zika Virus Infection. J Virol 2017 May 26;91(12).
27. Xiang T, Yang G, Liu X, Zhou Y, Fu Z, Lu F, Gu J, Taniguchi N, Tan Z, Chen X, Xie Y, Guan F, Zhang XL: Alteration of N-glycan expression profile and glycan pattern of glycoproteins in human hepatoma cells after HCV infection. Biochim Biophys Acta 2017 Feb 13.
28. He DL, Damaris RN, Fu JL, Tu JX, Fu TD, Chen X, Yi B, Yang PF: Differential molecular responses of rapeseed cotyledons to light and dark reveal metabolic adaptations towards autotrophy establishment. Front Plant Sci 2016;7:988.
29. Jin Y, Wang J, Ye X, Su Y, Yu G, Yang Q, Liu W, Yu W, Cai J, Chen X, Liang Y, Chen Y, Wong BH, Fu X, Sun H: Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins. Br J Cancer 2016 Mar 1;114(5):532-44.
30. Wang Z, Han QQ, Zhou MT, Chen X, Guo L: Protein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph, and quantitative proteomics. J Basic Microbiol 2016 Jan 15.
31. Zhou MT, Qin Y, Li M, Chen C, Chen X, Shu HB, Guo L: Quantitative Proteomics Reveals the Roles of Peroxisome-associated Proteins in Antiviral Innate Immune Responses. Mol Cell Proteomics 2015 Sep;14(9):2535-49.
32. Li HY, Zhang LK, Zhu XJ, Shang J, Chen X, Zhu Y, Guo L: Analysis of EV71 infection progression using triple-SILAC-based proteomics approach. Proteomics 2015 Aug 26.
33. He XM, Zhu GT, Zhu YY, Chen X, Zhang Z, Wang ST, Yuan BF, Feng YQ: Facile Preparation of Biocompatible Sulfhydryl Cotton Fiber-Based Sorbents by "Thiol-ene" Click Chemistry for Biological Analysis. ACS Appl Mater Interfaces 2014 Oct 10.
34. Wu X, Tian L, Li J, Zhang Y, Han V, Li Y, Xu X, Li H,
Chen X, Chen J, Jin W, Xie Y, Han J, Zhong CQ: Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 2012 Dec;11(12):1640-51.35. Deng M, Li F, Ballif BA, Li S, Chen X, Guo L, Ye X: Identification and functional analysis of a novel cyclin e/cdk2 substrate ankrd17. J Biol Chem 2009, 284:7875-7888.
36. Zhou Z, Fan JB, Zhu HL, Shewmaker F, Yan X, Chen X, Chen J, Xiao GF, Guo L, Liang Y: Crowded cell-like environment accelerates the nucleation step of amyloidogenic protein misfolding. J Biol Chem 2009, 284:30148-30158.
